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. 2016 Jun 22;107(8):1101–1109. doi: 10.1111/cas.12977

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© 2016 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Immunofluorescent staining for intranodal macrophages adhered to lymphatic vessels of human metastatic lymph nodes. Nuclei of cells in metastatic lymph nodes were stained with DAPI (a). Images of lymphatic vessels stained with anti‐podoplanin antibody (green) (b), macrophages stained with anti‐CD163 antibody (red) (c), and merged images (d) are shown. White arrows indicate macrophages surrounding and contacting lymphatic vessels. (e) Merged images of the lymphatic vessels stained with anti‐podoplanin antibody (green) and anti‐CD163 antibody (red) are shown. White arrows indicate macrophages. (f) The same section was stained with anti‐podoplanin (green) and anti‐lymphatic vessel endothelial hyaluronan receptor (LYVE)1 antibody (red). Podoplanin‐positive cells are coexpressing LYVE1.