Fig 5. Timp-3 deficiency and transgenic chondrocyte-specific overexpression increase trabecular TRAP staining.
Representative images of TRAP stained sections from WTB6 (A-D), KO (E-H), WTF1 (I-L) and Tg (M-P) mice demonstrating TRAP activity in growth plate, trabecular and cortical bone. Staining show that TRAP activity is significantly higher in KO and Tg sections compared with their WT groups. (Q) TRAP activity was quantified to produce osteoclast surface/bone surface and osteoclast number/bone surface. Two-sample t-test was used to compare means between KO and WTB6, and between Tg and WTF1. Normality of variance assumption were not violated for any experimental group. Homogeneity of variance osteoclast surface/bone surface between KO and WTB6 was violated (p >0.05) and thus for these groups Kruskal-Wallis test was performed. Group sizes were n = 4. Data are mean ± SEM. Statistical comparisons: * denotes p ≤ 0.05.