Table 1. Mean value of morphometric parameters from the 2D and 3D analysis representing trabecular and cortical mass and architecture of WTB6, Timp-3 KO, WTF1 and Timp-3 Tg mice at 8 weeks of age.
Morphometric index | WTB6 | KO | P value | WTF1 | Tg | P value |
---|---|---|---|---|---|---|
n = 5 | n = 6 | WTB6 vs KO | n = 5 | n = 6 | WTF1 vs Tg | |
Tibial length (mm) | 18.11 ± 0.02 | 16.45 ± 0.06 | <0.001 | 17.33 ± 0.05 | 17.32 ± 0.02 | NS |
Trabecular parameters | ||||||
Trabecular BMD g/cm3 | 0.304 ± 0.01 | 0.263 ± 0.01 | <0.05 | 0.409 ± 0.02 | 0.350 ± 0.01 | NS |
Total volume (micro-m3) x 109 | 2.34 ± 0.12 | 1.74 ± 0.09 | <0.01 | 1.99 ± 0.09 | 2.00 ± 0.07 | NS |
Bone volume (micro-m3) x 108 | 5.45 ± 0.50 | 3.88 ± 0.18 | <0.05 | 5.63 ± 0.59 | 4.78 ± 0.42 | NS |
Trabecular separation (mm) | 0.14 ± 0.00 | 0.12 ± 0.00 | <0.05 | 0.14 ± 0.01 | 0.15 ± 0.01 | NS |
Cortical parameters | ||||||
Cortical BMD g/cm3 | 0.505 ± 0.04 | 0.334 ± 0.03 | <0.01 | 0.592 ± 0.03 | 0.554 ± 0.05 | NS |
Group sizes for were n = 5 for WT littermates and n = 6 for Timp-3 KO and Tg mice, except for n = 4 for all groups for BMD. Two-sample t-test was used to compare means between KO and WTB6, and between Tg and WTF1. Normality or the homogeneity of variance assumption were not violated (p ≥ 0.05). Data are mean ± SEM.