Figure 2. Chase component of the [³⁵S]Met labeling of basophils.

Purified human basophils were incubated for 24 h with 5 ng/ml, and the cells were pulsed with [³⁵S]Met for 18 h, washed, and placed back into similar medium without [³⁵S]Met. In 2 experiments, conditions included ±100 μM lactacystin A. Samples at 0 and 4 h were removed and lysed for IP. (A) An example of the Western blot and autoradiographs. (B) Average of the experiments.