Skip to main content
. 2016 Feb 29;100(3):625–635. doi: 10.1189/jlb.5A1215-580R

Figure 4. ADR treatment results in the emergence of indolent and quiescent tumor dormancy.

Figure 4.

(A) MMC tumor cells were treated with 3 daily doses of ADR (1 µM for 2 h) and then remained untreated for 3 wk. The frequency of viable MMC cells was determined by quantifying FVS− cells using flow cytometry. (B) At wk 1 and 3 post-treatment, adherent and viable tumor cells were counted by trypan blue exclusion. (C and D) MMC cells were treated for 3 consecutive d with ADR (1 µM, 2 h) or left untreated. Three weeks later, ADR-treated and untreated MMC cells were stimulated with IFN-γ (50 ng/ml) for 12–16 h to induce the expression of PD-L1. (C) Emergence of Ki67 was determined in control MMC cells (Untreated), as well as ADR-treated cells (+ADR) ± IFN-γ stimulation. (D) The expression of PD-L1/cell was calculated by dividing PD-L1 MFI by the frequency of Ki67− cells in ADR-treated and untreated MMC cells ± IFN-γ stimulation. Data represent 3 independent experiments and means ± sem.