Skip to main content
. 2016 Aug 12;12(8):e1005802. doi: 10.1371/journal.ppat.1005802

Fig 2. Impβ binding to HBV is affected by ionic strength and requires the arginine rich C-terminus.

Fig 2

Elution profiles of human importin β1 (Impβ, 5.3 μM) with HBV capsids (7.9 μM dimer). Samples of Impβ with (a) empty Cp183 capsids, 0.15 M NaCl; (b) empty Cp183 capsids, 0.25 M NaCl; (c) Cp183 capsids containing E. Coli RNA, 0.15 M NaCl: (d) empty P-Cp183 capsids, 0.15 M NaCl; (e) empty P-Cp183 capsids, 0.25 M NaCl; (f) P-Cp183 capsids containing E. Coli RNA, 0.15 M NaCl: (g) empty Cp149 capsids, 0.15 M NaCl. Samples were eluted through a Superose 6 column equilibrated with 20 mM TrisHCl pH7.4 and NaCl at the came concentration used for sample incubation and at a flow rate of 0.5 ml/min. Chromatographs are overlaid by images of Coomassie-stained SDS-PAGE of the corresponding fractions.