Fig 6.

Effect of immunosuppressants on the histological structure using hematoxylin-eosin (A-D) and Masson (E-H) staining in glomerulus obtained from xenograft mice model. Kidney was removed from Huh 7 cells implanted in athymic mice. Cells (10x106) were implanted at dorsal level in athymic mice, and 24 hours the treatments were initiated using oral administration following the dosage described in Material and Methods. Control: A and E, Everolimus: B and F, Sirolimus: C and G, and Tacrolimus: D and H. Animals were sacrificed when one tumor size reached 15 mm. Hematoxylin-eosin and Masson staining were carried out using standard procedures. The images are representative of four independent experiments. Original magnification 60x.