Table 1. Recommended tissue fixation and TDE concentrations for clearing of various tissue types.

Tissue-type	Standard clearing protocol		Rapid clearing protocol
	o/n fixation, 3h - o/n clearing		1h fixation, 1h clearing
	CFP / GFP	YFP / dsRed	CFP / GFP	YFP / dsRed
Root
Shoot	70% TDE	70% TDE	40% TDE	50% TDE
Cotyledons
Leaves	50% TDE	50% TDE	20% TDE	20% TDE
Anthers
Siliques	50% TDE	70% TDE	40% TDE	50% TDE
Isol. ovules

Depending on the tissue type of choice and fixation time, the table gives recommendations for TDE concentrations (in % v/v in H2O) for optimal clearing while at the same time preserving tissue integrity.