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. 2016 Aug 12;12(8):e1006253. doi: 10.1371/journal.pgen.1006253

Fig 6. Representative RNAP III C1-E850K mutant exhibits increased elongation.

Fig 6

A) Schematic of 3'-tailed templates used for promoter-independent transcription by FLAG-purified RNAPs III (see S2 Fig for establishment of assay). The template design on the left was used for panel B and the design on the right was used for panel C. FL = full length 300 nt transcript resulting from termination at 12T, RO = run-off transcript. B) Time course of elongation by purified RNAPs III C1-Wt and C1-E850K as indicated above the lanes. C) RNAP III was stalled at position +22 followed by a synchronized chase time course of transcription elongation (see text). (The blemish between lanes 5 & 6 is not a transcription signal.)