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. 2016 Aug 12;12(8):e1006253. doi: 10.1371/journal.pgen.1006253

Fig 8. RNAP III C1 mutants recognize and read through normal terminators.

Fig 8

A) Schematic of the tRNA reporter allele used for RT detection in strain yAS76, illustrating the transcripts produced from the 5T terminator and the 8T failsafe terminator. B-D) Northern blots. B) sup-tRNA intron-specific probe ("tRNA-Mser-int" see DNA oligos, Table 4); the RT transcript product resulting from termination at the failsafe 8T terminator, and pre-tRNA products resulting from termination at the 5T terminator, are indicated to the left. The intron-containing pre-tRNA processing species are indicated to the right. C) A readthrough-specific probe (equimolar mixture of three tRNA-LysCUU-RT oligo-DNAs, Methods) complementary to the region downstream of the natural terminator of tRNA-LysCUU gene, detects readthrough termination of endogenous tRNA gene (see text). D) A tRNALysCUU intron-specific probe (tRNA-LysCUU-int, Methods) of the same blot as in B (see text).