Table 4. DNA oligos.

C1-XhoI-Fwd	5’-TCAAGTTACTCGAGATGAAAGATCCTATTGATGATCAGG-3’
C1-NcoI-Rev	5’-ATTGTAGGGACCGCATACACATTCATTAAATCTTAAGGTACGCCATGGTC-3’
C1-NcoI-Fwd	5’ACTCCATAAGTTATCAATCATGGCTCACTTGGTAAAGGTACGACCATGGC-3’
C1-BstAp-Rev	5’TCAGTCACCCTGATAATGTTCTCAACAGATTTTCTTTTCGCAACGGGTGCA-3’
C1-BstAPI-Fwd	5’-ATGAATTTGCACCCGTTGCGAAAAGAAAATCT-GTTGAGAACA-3’
C1-XmaI-Rev	5’-TAGATCTACCCGGGTTAAGCCGTAATTTCCAATTGATG-3’
Rpc2_AscI_Fwd	5’- ATTAA GTAAGGCGCGCCATGGGGGTAAATACTGCC-3’
Rpc2_CHF_BglII-Rev	5’ATCGATAGATCTTCACTTATCGTCGTCATCCTTGTAATCGTGGTGATGGTGATGATGATACTTAAATTCGTCTTC -3’
C1-XhoI-Flag-Fwd	5’CAAGTCTCGAGATGGACTACAAAGACGATGACGACAAGAAAGATCCTATTGATGATCAGG-3’
TspR1 tailed template	5’ATCGCACTCACAGACCTGCAGTGAAGCGGACACAACCAGAGCAGGAAACTCCTAGCACAACTTTCATAGACAGCTCTGACTCTGGCTAGCTGACTTACACTTTACGATAGATGTACAGCTGCAGTCACTATGTCCGAGCTTTCAAGGAGTAGACTTCAGTCCTGCATGCTAGTCACCTTTCGGGCTCTGTCCGCGCAGGTTCAGATCCTGCTGGCGTTTGAAGATCTTCAGAGCTCGGTCAGAAAGGCCGGGCTCGGATTTGAGGTCTCCGTCACCAGCAGGATCCGAACCAATCAAGACTTTAATACAGGATTGAAGTCTAACTTTTTTTTTTTTGCCGCGAGTACCTGATCGCTCGATCCGTACGTACTAGGATCCTAGAGTCTTCCAGGAATCGGCATCCCGGGTACCATCG-3’
TspR1 tailed template (for walking)	5’ATCGCACTCACAGACCTGCAGTGAAGCGGACACAACCAGAGCAGGAATTTCCTAGCACAACTTTCATAGACAGCTCTGACTCTGGCTAGCTGACTTACACTTTACGATAGATGTACAGCTGCAGTCACTATGTCCGAGCTTTCAAGGAGTAGACTTCAGTCCTGCATGCTAGTCACCTTTCGGGCTCTGTCCGCGCAGGTTCAGATCCTGCTGGCGTTTGAAGATCTTCAGAGCTCGGTCAGAAAGGCCGGGCTCGGATTTGAGGTCTCCGTCACCAGCAGGATCCGAACCAATCAAGACTTTAATACAGGATTGAAGTCTAACTTTTTTTTTTTTGCCGCGAGTACCTGATCGCTCGATCCGTACGTACTAGGATCCTAGAGTCTTCCAGGAATCGGCATCCCGGGTACCATCG-3’
tRNA-LysCUU-RT	5’- TCTTCATACACTAGCATTTAATG-3’
	5’-CAGTATAAGGAAAAGTGGTGTAT-3’
	5’-ACATAGCAAGTGTGTTAACCATT-3’
tRNA-LysCUU-int	5’-CTTCTGATACCATTCGTAAGAGTC-3’
tRNA-Mser-cRT	5’-CCTGCTGGTGACGGAGACC-3’
	5’-TTCTAGTCTTGATTGGTTCA-3’
	5’-AGTTAGACTTCAATCCTG-3’
tRNA-Mser-int	5’-CATTAAATGACTAGAATACAGGATTGAAGTC-3’