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. 2016 Aug 12;11(8):e0161347. doi: 10.1371/journal.pone.0161347

Fig 4. qPCR validation of different transcription factors and genes related to the Wnt signaling.

Fig 4

(A) The relative mRNA expression levels of different transcription factors, and (B) genes associated with the Wnt signaling were determined with the 2-(ΔΔCt) method normalized with the geometric mean of the five housekeeping genes. Bars represent the mean ratio of target gene expression in five patients’ fibroblasts compared to five unrelated healthy individuals. qPCR was performed in triplicate, and the results are expressed as mean ± SEM. Statistical significance was calculated with one sample t test (*p<0.05, **p<0.01 and ***p<0.001).