. 2016 Aug 12;10(8):e0004791. doi: 10.1371/journal.pntd.0004791

Table 2. Drug sensitivity profile of Tb427WT cells expressing either the wild-type F₁ subunit γ gene, or the same gene with mutation S284^*, either exposed to ethidium bromide or ISM, or not exposed to any trypanocide.

All data are the mean of three independent experiments and SEM.

	WT ATPase γ	S284^* ATPase γ Unexposed		S284^* ATPase γ Isometamidium Exposed			S284^* ATPase γ Ethidium Bromide Exposed
Compound	Mean EC₅₀ (nM)	Mean EC₅₀ (nM)	Resistance Factor vs. WT ATPase γ	Mean EC₅₀ (nM)	Resistance Factor vs. WT ATPase γ	Resistance Factor vs. Unexposed	Mean EC₅₀ (nM)	Resistance Factor vs. WT ATPase γ	Resistance Factor vs. Unexposed
Isometamidium	0.31 ± 0.06	31.9 ± 6.1	104^*	172 ± 59	560^***	5.4^***	162 ± 46	529^***	5.1^***
Ethidium Bromide	1.49 ± 0.21	554 ± 50	372^***	361 ± 16	242^***	0.65	322 ± 36	216^***	0.58
Diminazene	20.3 ± 0.8	108 ± 6.4	5.3^**	132 ± 24	6.5^***	1.2	136 ± 26	6.7^***	1.3
Pentamidine	2.8 ± 0.3	7.22 ± 0.66	2.6^**	2.3 ± 0.3	0.82	0.32	2.38 ± 0.73	0.84	0.33
Oligomycin	270 ± 33	1410 ± 90	5.2^***	872 ± 282	3.2^**	0.62	1180 ± 170	4.4^***	0.83

Asterisks represent P-values for statistically significant resistance, as calculated using a one-tailed Student’s t-test:

*, P-value <0.05;

**, P-value <0.01;

***, P-value <0.001.

Table 2. Drug sensitivity profile of Tb427WT cells expressing either the wild-type F1 subunit γ gene, or the same gene with mutation S284*, either exposed to ethidium bromide or ISM, or not exposed to any trypanocide.