Figure 3. Nkx2-5 associates with the 5’-3’ exonuclease Xrn2.
(A) Co-immunoprecipitates derived using the indicated antibodies from nuclear extracts of E12.5 hearts and aliquots (6%) of the input proteins were analyzed by Western blotting. (B) Xrn2 and Nkx2-5 deletion mutants were transfected into C3H10T1/2 cells. Co-immunoprecipitates derived using the M2 antibody and aliquots (7%) of the input proteins were analyzed by Western blotting. Schematic presentation of Xrn2 and its deletion mutants is shown at the right panel. (C) Nkx2-5 and Xrn2 deleting mutants were transfected into C3H10T1/2 cells. Co-immunoprecipitates derived using the HA antibody and aliquots (7%) of the input proteins were analyzed by Western blotting. Schematic presentation of Nkx2-5 and its deletion mutants is shown at the right panel. (D) Co-immunoprecipitates derived using the indicated antibodies from nuclear extracts of E12.5 hearts exposed to 20 µg/ml EtBr or 50 µg/ml RNaseA as well as aliquots (6%) of the input proteins were analyzed by Western blotting. (E) Summary of interacting regions between Nkx2-5 and Xrn2. HD, homeodomain; Xrn, Xrn domain; ZF, zinc finger.