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. Author manuscript; available in PMC: 2016 Aug 15.
Published in final edited form as: Oncogene. 2016 Feb 15;35(32):4235–4243. doi: 10.1038/onc.2015.488

Figure 4. Monoclonal antibody clone 83-7 against insulin receptor (83-7) downregulated insulin receptor, inhibiting insulin-stimulated signaling and growth in TamR cells.

Figure 4

(A) MCF-7L and T47D cells were serum starved and pre-treated with 2 μg/mL of 83-7 overnight before treating with either 5nM IGF-I, 10nM IGF-II or 10nM insulin for 15 minutes. Whole cell lysate were collected, separated by SDS-PAGE and subjected for indicated immunoblotting analyses. Anchorage-independent growth assay was carried out on (B) MCF-7L and T47D cells treated with 2 μg/mL of 83-7 without or with either 10nM IGF-I or 10nM insulin. Colonies formed were counted 14 days for MCF-7Ls and 21 days for T47D cells after first plated. Two-way ANOVA with Bonferroni comparison was performed to identify significance among untreated vs. treated groups. *, p<0.05; **, p<0.01.