Table 2. Ramp2−/− Tg adults develop left ventricle dilatation and declining heart function.
Echocardiography analysis from Ramp2+/+, Ramp2+/−, and Ramp2−/− mice with and without the transgene.
| Echo Parameters | ntg | Tg (Cdh5-Ramp2) | |||
|---|---|---|---|---|---|
| Ramp2+/+ | Ramp2+/− | Ramp2+/+ | Ramp2+/− | Ramp2−/− | |
| Mouse Number | 10 | 4 | 7 | 4 | 7 |
| Mouse Age (wks) | 26.7±1.3 | 26.8±2.0 | 29.3±2.3 | 26.3±2.2 | 29.7±1.6 |
| Heart Rate (BPM) | 624±15 | 578±38 | 574±50 | 614±55 | 552±21 |
| LV Vol, d (μL) | 35.6±4.7 | 32.0±3.9 | 34.1±6.7 | 27.9± 5.9 | 73.4±9.5*,†,‡,§ |
| LV Vol, s μL) | 7.6±1.6 | 5.0±1.4 | 7.0±2.7 | 5.4±2.4 | 23.1±3.5*,†,‡,§ |
| CO (mL/min) | 16.5±2.1 | 15.4±1.2 | 16.1±3.2 | 13.2±0.9 | 27.0±4.8* |
| EF (%) | 81.2±3.0 | 85.1±2.0 | 82.4±3.6 | 83.3±5.1 | 68.3±2.3*,†,‡,§ |
| FS (%) | 51.6±2.7 | 52.9±2.2 | 51.1±4.0 | 52.4±6.5 | 37.9±1.8*,‡ |
| IVS, s (mm) | 1.13±0.05 | 1.16±0.06 | 1.12±0.07 | 1.07±0.07 | 1.08±0.07 |
| IVS, s (mm) | 1.72±0.06 | 1.74±0.06 | 1.73±0.07 | 1.68±0.13 | 1.60±0.07 |
| LVID, d (mm) | 2.94±0.20 | 2.88±0.14 | 2.90±0.22 | 2.70±0.23 | 4.03±0.22*,†,‡,§ |
| LVID, s (mm) | 1.52±0.17 | 1.36±0.13 | 1.45±0.21 | 1.32±0.27 | 2.50±0.14*,†,‡,§ |
| LVPW, d (mm) | 1.54±0.10 | 0.96±0.07 | 1.22±0.13 | 1.08±0.30 | 0.87±0.06 |
| LVPW, s (mm) | 1.74±0.13 | 1.72±0.04 | 1.76±0.08 | 1.60±0.20 | 1.40±0.08 |
BPM, beats per minute; LV, left ventricle; d, diastole; s, systole; CO, cardiac output; EF, ejection fraction; FS, fractional shortening; IVS, interventricular septal; LVID, left ventricle internal diameter; LVPW, left ventricle posterior wall. Data represented as averages ± SEM with significance of p<0.05 determined by one-way ANOVA with Tukey’s Multiple Comparison. Significant compared to:
*
Ramp2+/+ ntg,
†
Ramp2+/− ntg,
‡
Ramp2+/+ Tg,
§
Ramp2+/− Tg.