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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: Traffic. 2016 Jul 11;17(9):1027–1041. doi: 10.1111/tra.12418

Figure 7. Expression of the p50RhoGAP Sec14 homology domain (NTD) inhibits Ag-stimulated β-Hex-pHl exocytosis.

Figure 7

RBL-2H3 cells were transfected with β-Hex-pHl and either full length (FL) or the N-terminal domain (NTD) of p50RhoGAP fused to mCherry and sensitized with anti-DNP IgE before harvest. Fluorescence was monitored by spectroscopy. Cells were treated with 2 μM cytoD or vehicle (DMSO, -cytoD) for 5 minutes before exocytosis was stimulated with 100ng/ml DNP-BSA. The bar graph shows integrated fluorescence values expressed as a ratio (NTD/FL) to quantify rescue of NTD-mediated inhibition by cytoD. The average of four experiments is shown at right for t=300 s, error bars are standard deviations, and the p-value was calculated using the unpaired t-test.