Table II.
Quantitative PCR genes and primer sequences.
| Genes | Accession number | Primer sequences (5′ to 3′) | |
|---|---|---|---|
| ALP | NM_000478.4 | Forward | ACTGGTACTCAGACAACGAGA |
| Reverse | ACGTCAATGTCCCTGATGTTATG | ||
| BMP-2 | NM_001200.2 | Forward | ACTACCAGAAACGAGTGGGAA |
| Reverse | GCATCTGTTCTCGGAAAACCT | ||
| OC | NM_000234.2 | Forward | CACTCCTCGCCCTATTGGC |
| Reverse | CCCTCCTGCTTGGACACAAAG | ||
| Col-1 | NM_000088.3 | Forward | GAGGGCCAAGACGAAGACATC |
| Reverse | CAGATCACGTCATCGCACAAC | ||
| RUNX2 | NM_001024630.3 | Forward | TGGTTACTGTCATGGCGGGTA |
| Reverse | TCTCAGATCGTTGAACCTTGCTA | ||
| TGF-β1 | NM_000660.5 | Forward | CAATTCCTGGCGATACCTCAG |
| Reverse | GCACAACTCCGGTGACATCAA | ||
| GAPDH | NM_002046.5 | Forward | ACAACTTTGGTATCGTGGAAGG |
| Reverse | GCCATCACGCCACAGTTTC | ||