Figure 5.

HSP analysis of cells cotransfected with green and red fluorescent-labeled proteins. (A) Dual-color HSP brightness plot: The gray shaded zone represents all possible brightness values that identify the presence of protein complexes carrying both green and red labels. Samples I and II have the same average amount of green-labeled proteins per diffusing complex, but sample II contains more red-labeled proteins per diffusing complex than sample I. The green line represents the limit where complexes carrying both colors are absent. (B) The HSP brightness values for GFP-Epsin1 and CLC-mCherry (red solid circles) and for GFP-Epsin1 and CAV1-mCherry (blue solid triangles). To see this figure in color, go online.