Table 1.
Tracking Data for All Cells, Showing Means ± SD
| Parameter | E. coli AW405a | E. coli HCB1737 | E. coli HCB1737 Cephalexin | E. coli HCB1737 Swarm | B. subtilis DS9540 | B. subtilis DK2002 | Enterococcus V4051 |
|---|---|---|---|---|---|---|---|
| Number of cells | 35 | 137 | 155 | 274 | 117 | 139 | 64 |
| Number of cells that tumbled | 35 | 105 | 119 | 41 | 71 | 119 | 59 |
| Run speed (μm/s) | 14.2 ± 3.4 | 19.9 ± 5.6 | 23.0 ± 6.7 | 19.8 ± 6.9 | 23.3 ± 7.3 | 24.8 ± 8.2 | 20.3 ± 4.7 |
| Tumble speed (μm/s) | 14.3 ± 4.8 | 15.7 ± 5.3 | 16.7 ± 9.6 | 16.6 ± 9.3 | 15.8 ± 5.2 | 14.9 ± 3.9 | |
| Angular speed while running (°/point)b | 5.9 ± 3.2 | 10.8 ± 4.3 | 10.2 ± 4.1 | 3.9 ± 3.8 | 7.3 ± 5.0 | 11.5 ± 2.7 | 13.2 ± 5.2 |
| Angular speed while tumbling (°/point) | 23.5 ± 12.2 | 33.2 ± 7.5 | 30.4 ± 5.9 | 27.2 ± 7.0 | 27.6 ± 8.3 | 29.0 ± 5.0 | 28.0 ± 4.7 |
| Run interval (s) | 0.86 ± 1.18 | 2.9 ± 4.9 | 1.6 ± 2.9 | 7.1 ± 5.8 | 2.3 ± 2.0 | 0.91 ± 0.72 | 0.91 ± 1.4 |
| Tumble interval (s) | 0.14 ± 0.19 | 0.32 ± 0.18 | 0.28 ± 0.15 | 0.19 ± 0.12 | 0.17 ± 0.10 | 0.27 ± 0.14 | 0.33 ± 0.19 |
| Change in direction from run to run (°) | 68 ± 36 | 60.0 ± 29.2 | 64.7 ± 28.0 | 50.7 ± 32.8 | 51.8 ± 26.9 | 61.4 ± 25.3 | 68.0 ± 26.6 |
| Change in direction during runs (°) | 23 ± 23 | 17.4 ± 7.9 | 17.7 ± 10.3 | 23.8 ± 10.9 | 24.8 ± 13.7 | 23.5 ± 12.6 | 20.8 ± 10.5 |
| Tumble frequency (s−1) | ∼1 | 0.72 ± 0.60 | 1.0 ± 0.6 | 0.11 ± 0.34 | 0.50 ± 0.61 | 0.90 ± 0.62 | 1.2 ± 0.6 |
| Number of events (runs, tumbles) | 856, 754 | 898, 794 | 504, 232 | 397, 299 | 851, 749 | 505,467 | |
| Total tracking time (s) | 1094 | 828 | 2213 | 552 | 823 | 399 | |
| Body diameter (μm) | 0.73 ± 0.1 (89) | 1.0 ± 0.1 (103) | 1.1 ± 0.08 (24) | 1.2 ± 0.1 (68) | 1.2 ± 0.1 (117) | 1.3 ± 0.11 (90) | |
| Body length (μm) | 1.9 ± 0.5 (89) | 5.0 ± 1.9 (103) | 7.9 ± 4.8 (24) | 6.9 ± 2.3 (68) | 5.0 ± 1.7 (117) | 4.4 ± 2.1 (90) | |
| Number of filaments | 3.3 ± 0.9 (39) | 3.4 ±1.7 (40) | 3.9 ± 0.9 (7) | ∼26c | 5.8 ± 2.1 (22) |