Figure 3.

RDC and PRE data of bound and unbound K₂. (A) A plot of RDC values. The diagram at the top of the figure shows the location of the various segments in the K₂ sequence. (B) A plot of the PRE caused by Cys24-MTSL. The intensity of the signal is normalized to that in the unbound spectrum. The dashed line represents the theoretical intensity of a random-coil structure (38). In (A) and (B), the black bars represent free K₂ and the gray bars bound K₂. (C) Protection factors of amide residues. The exchange rates of backbone amide groups were converted to protection factors. Black circles represent free K₂ and gray symbols bound K₂. Circles represent calculated protection factors and squares represent protection factors that could not be accurately quantified.