Figure 5.

Lysine residues in the K-segment are key to SDS micelle binding. (A) PRE of signals in the ¹⁵N-HSQC in the presence of MnCl₂. Signal intensities were normalized to those in the absence of the paramagnetic agent. Black bars represent 0.25 mM MnCl₂ and gray bars 1 mM MnCl₂. (B) PRE of signals in the ¹⁵N-HSQC in the presence of 5-doxyl stearate. Signal intensities were normalized to those in the absence of the paramagnetic agent. The diagram at the top of the figure shows the location of the various segments in the K₂ sequence.