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. 2016 Jul 21;7(2):236–248. doi: 10.1016/j.stemcr.2016.06.010

Figure 4.

Figure 4

Validation of RBP2 as a Direct Target Gene of miR-34a

(A–C) Quantitative real-time PCR (A) and western blotting (B and C) analysis of the effects of miR-34a on RBP2 expression.

(D) Predicted binding sites of miR-34a in the 3′ UTR of RBP2-WT mRNA (underlined part indicates mutated base sequences in the 3′ UTR of RBP2-MT).

(E) Schematic showing the constructed luciferase reporter system containing the binding sites of miR-34a.

(F) Luciferase activity of cells with miR-34a overexpression in the RBP2-WT or RBP2-MT group.

RBP2, retinoblastoma binding protein 2; RBP2-WT, wild-type RBP2 mRNA; RBP2-MT, mutant-type RBP2 mRNA. Data represent the means ± SD of three independent experiments. ∗p < 0.05 versus the NC group.