Osx+ Cell Ablation Causes B lymphopenia and an Increase in Monocytes and Macrophages
(A) Flow cytometric measurement of bone marrow cellularity and multiple mature lineages in OsxCre;iDTR mutants compared with control littermates.
(B) White blood cell, red blood cell, and platelet counts in the peripheral blood of OsxCre;iDTR mutants versus controls.
(C) Enumeration of different progenitor subtypes in the bone marrow.
(D) Examination of the spleen as a major site for B lymphopoiesis and extramedullary hematopoiesis showed a similar increase in Mac1+Gr1+ myeloid cells and attenuated mature B cell number.
Experiment repeated 2–3 times; n = 4–12/group. ∗p < 0.05, ∗∗∗p < 0.001. Error bars represent ± SEM.