Figure 3.

Osx⁺ Cell Ablation Causes B lymphopenia and an Increase in Monocytes and Macrophages

(A) Flow cytometric measurement of bone marrow cellularity and multiple mature lineages in OsxCre;iDTR mutants compared with control littermates.

(B) White blood cell, red blood cell, and platelet counts in the peripheral blood of OsxCre;iDTR mutants versus controls.

(C) Enumeration of different progenitor subtypes in the bone marrow.

(D) Examination of the spleen as a major site for B lymphopoiesis and extramedullary hematopoiesis showed a similar increase in Mac1⁺Gr1⁺ myeloid cells and attenuated mature B cell number.

Experiment repeated 2–3 times; n = 4–12/group. ^∗p < 0.05, ^∗∗∗p < 0.001. Error bars represent ± SEM.