Fig. 4.

Example of a result page produced by the User Data Annotation function. a Output VCF file generated by the function, in which 9 information fields are newly added as indicated by the blue asterisk. b Most frequently observed cases when compared mutation consequences between major and canonical/principal isoforms using somatic mutations of TCGA STAD data. For each case, the TCGA sample barcode, gene symbol, chromosomal location, base change, and respective amino acid changes in the major and canonical isoforms are shown