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. 2016 Aug 12;16:186. doi: 10.1186/s12866-016-0792-6

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Histopathology of duodenum of broilers following chicken intestinal loops inoculated with C. perfringens. H&E-stained paraffin section of a duodenum villus. a: Enterocytes and villi appeared to be well organized in control loops with only TPG broth. b-d: Loops with C. perfringens inoculations, where the cell membranes have broken down and there is an appearance of coagulation necrosis. Large numbers of C. perfringens lining the damaged villi are visible in c and d. Arrows point out C. perfringens. Size bars/magnifications: a and b = 20 μm/X 100; c and d = 5 μm/X400