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. 2016 Aug 9;7(2):167–176. doi: 10.1016/j.stemcr.2016.07.006

Figure 1.

Figure 1

Matrix-Integrin Binding Activates FAK Signaling Upstream of AKT

(A) Immunofluorescence of hESCs cultured on fibronectin for 24 hr and stained with antibodies against OCT4 and pFAKY397. Scale bar, 50 μm.

(B) Immunoblot of pFAKY397, FAK, pAKTS473, and AKT in hESCs stably cultured on fibronectin.

(C) Immunoblot of pFAKY397, FAK, pAKTS473, and AKT in hESCs 1 hr after being plated in the following conditions, on: fibronectin (CTL); fibronectin plus 10 μg/mL of β1-integrin blocking antibody (MAB13); non-integrin activating Poly-L-Lysine substrate or fibronectin plus 2 μM PF562271 (FAKi).

(D) Densitometry of immunoblots for pFAKY397/FAK ratio and pAKTS473/AKT ratio for the conditions in (C). Data represent mean + SEM (n = 3 experiments). ∗p < 0.05 relative to CTL.

(E) Integrin signaling cascade and points of inhibition.

See also Figure S1.