Table 1.
Metabolic profiles of E.coli mutants cultivated in NBS medium supplemented with 10 g/L glucose under anaerobic condition
| Strains | Fermentation time (h) | CDW (g/L) | Glucose consumed (mM) | Succinate (mM) | Succinate yield (mol/mol) | Acetate (mM) | Pyruvate (mM) | Lactate (mM) | Specific growth rate during 0–24 h (g/g·h) | Specific glucose uptake rate during 0–24 h (g/g·h) |
|---|---|---|---|---|---|---|---|---|---|---|
| ZTK | 42 | 1.03 ± 0.07 | 54.26 ± 0.26 | 54.91 ± 0.29 | 1.01 ± 0.006 | 26.10 ± 1.49 | – | – | 0.042 ± 0.001 | 0.44 ± 0.01 |
| ZTK (pZY02E) | 84 | 0.75 ± 0.03 | 53.33 ± 0.45 | 59.56 ± 0.40 | 1.12 ± 0.004 | 30.1 ± 0.03 | – | – | 0.021 ± 0.002 | 0.29 ± 0.01 |
| ZTK (pZY02) | 72 | 0.79 ± 0.01 | 54.26 ± 0.26 | 64.08 ± 0.40 | 1.18 ± 0.006 | 28.18 ± 0.18 | – | – | 0.021 ± 0.001 | 0.33 ± 0.03 |
| WSA110 | 48 | 0.85 ± 0.01 | 53.89 ± 0.45 | 62.40 ± 0.38 | 1.16 ± 0.005 | 32.53 ± 0.62 | 1.50 ± 0.04 | – | 0.033 ± 0.0004 | 0.39 ± 0.03 |
| WSA134 | 48 | 0.83 ± 0.003 | 54.26 ± 0.26 | 65.80 ± 0.22 | 1.21 ± 0.002 | 31.48 ± 0.57 | – | – | 0.029 ± 0.001 | 0.37 ± 0.006 |
| WSA138 | 48 | 0.93 ± 0.008 | 54.44 ± 0.45 | 67.74 ± 0.36 | 1.24 ± 0.005 | 27.78 ± 0.56 | – | – | 0.035 ± 0.0008 | 0.43 ± 0.01 |
| WSA146 | 48 | 0.89 ± 0.05 | 53.33 ± 0.45 | 69.68 ± 0.26 | 1.31 ± 0.005 | 23.68 ± 0.28 | – | – | 0.032 ± 0.0007 | 0.40 ± 0.01 |
| WSA150 | 54 | 0.76 ± 0.008 | 51.48 ± 1.31 | 69.99 ± 1.55 | 1.36 ± 0.007 | 3.53 ± 0.04 | 1.44 ± 0.02 | 0.78 ± 0.06 | 0.031 ± 0.002 | 0.34 ± 0.03 |
| WSA152 | 48 | 0.75 ± 0.009 | 50.93 ± 0.26 | 66.75 ± 0.35 | 1.31 ± 0.001 | 1.74 ± 0.04 | 3.49 ± 0.11 | 0.49 ± 0.05 | 0.023 ± 0.002 | 0.38 ± 0.02 |
| WSA157 | 42 | 0.81 ± 0.014 | 51.67 ± 1.20 | 72.50 ± 1.59 | 1.40 ± 0.002 | 0.99 ± 0.03 | 1.71 ± 0.06 | 0.53 ± 0.09 | 0.032 ± 0.002 | 0.41 ± 0.01 |
| WSA159 | 48 | 0.69 ± 0.005 | 52.59 ± 0.26 | 80.56 ± 0.10 | 1.53 ± 0.006 | 1.36 ± 0.14 | 1.43 ± 0.04 | 0.85 ± 0.07 | 0.027 ± 0.002 | 0.39 ± 0.03 |
| WSA161 | 36 | 0.97 ± 0.02 | 50.56 ± 0.45 | 73.61 ± 0.70 | 1.46 ± 0.01 | 0.97 ± 0.17 | 0.96 ± 0.09 | 0.30 ± 0.03 | 0.051 ± 0.002 | 0.44 ± 0.03 |
| WSA163 | 60 | 0.72 ± 0.05 | 52.50 ± 0.26 | 80.72 ± 0.24 | 1.54 ± 0.01 | 1.34 ± 0.03 | 1.51 ± 0.02 | 0.96 ± 0.02 | 0.024 ± 0.005 | 0.28 ± 0.03 |
| WSA165 | 36 | 0.90 ± 0.03 | 52.59 ± 0.52 | 78.64 ± 0.79 | 1.50 ± 0.005 | 2.08 ± 0.05 | 2.00 ± 0.20 | – | 0.044 ± 0.002 | 0.44 ± 0.02 |
| WSA167 | 36 | 1.03 ± 0.02 | 53.33 ± 0.45 | 77.82 ± 0.55 | 1.46 ± 0.008 | 1.87 ± 0.06 | 2.66 ± 0.14 | – | 0.054 ± 0.002 | 0.54 ± 0.005 |
Bacteria were cultivated with 50 mL NBS medium in a 100 mL flask shaking at 220 rpm and 37 °C in anaerobic conditions with an initial OD600 of 0.6; “–” represents data were not measured in this work. Data are average values and standard deviations of triplicate experiments