Table 3.
Strains and plasmids used in this study
| Strains and plasmids | Relevant characteristics | Sources or references |
|---|---|---|
| Strains | ||
| ZTK | W1485, ΔptsG ΔldhA ΔpflB; kanr | Lab stock |
| WSA110 | ZTK, ΔlacZ::Ptrc-zwf243-gnd361 from C. glutamicum; kanr | This study |
| WSA134 | WSA110, PBJ23100-talB; PBJ23100-tktA; PBJ23100-pgl; kanr | This study |
| WSA138 | WSA134, PBJ23100-pepck; Δppc::PBJ23100-pepck from A. succinogenes; kanr | This study |
| WSA146 | WSA138, PBJ23100-sthA; kanr | This study |
| WSA150 | WSA146, ΔackA-pta; kanr | This study |
| WSA152 | WSA150, PBJ23100-galp; kanr | This study |
| WSA157 | WSA152, ΔlldD::PBJ23100-pyc from C. glutamicum; kanr | This study |
| WSA159 | WSA157, PBJ23100-dcuB; kanr | This study |
| WSA161 | WSA157, PBJ23100-dcuC; kanr | This study |
| WSA163 | WSA157, PBJ23100-dcuB; PBJ23100-dcuC; kanr | This study |
| WSA165 | WSA159, Δzwf243-gnd361-protein bind; kanr | This study |
| WSA167 | WSA163, Δzwf243-gnd361-protein bind; kanr | This study |
| DH5α | Cloning host | Lab stock |
| Plasmids | ||
| pZY02E | p15 ori; Ptrc-zwf-gnd of E.coli; Cmr | This study |
| pZY02 | p15 ori; Ptrc-zwf243-gnd361 of C. glutamicum; Cmr | Lab stock |
| pCU18 | pBR322 ori; Ampr | Lab stock |
| pCU18-zwf243-gnd361 | Ptrc-zwf243-gnd361 from pZY02, cat gene from pEL04, upstream and downstream fragments of lacz gene were cloned into pCU18; Ampr, Cmr | This study |
| pCU18-pyc | PBJ23100- pyc from C. glutamicum was cloned into pCU18; Ampr | This study |
| pCU18-ppc | Upstream and downstream fragments of ppc gene were cloned into pCU18; Ampr | This study |
| pCU18-cat-sacB | cat-sacB genes from pEL04 were cloned into pCU18-ppc; Ampr, Cmr | This study |
| pCU18-pepck | PBJ23100- pepck from A. succinogenes was cloned into pCU18-ppc; Ampr | This study |
| pEL04 | cat-sacB cassette; Cmr | [41] |
| PTKSS | p15A replication, Tetr, I-SceI restriction sites; Cmr, Tetr | [49] |
| PTKRED | pSC10 replication, temperature sensitive replication origin, ParaBAD-driven I-SceI gene, red recombinase expression plasmid, lac-inducible expression; Spcr | [49] |
kan kanamycin; Amp ampicillin; Cm chloramphenicol; Tet tetracycline; Spc spectinomycin; r resistance