Table 2.
| Condition | Line | Experimental Setup Mice Were Generated from: |
|
|---|---|---|---|
| 1 ESC into 2C Embryo | 5 ESCs into Morula | ||
| D serum, C serum | 3.2 | – | 1/1 |
| 3.4 | – | 1/1 | |
| D 2i, C 2i | 2.2 | 3/3 | 3/3 |
| 2.5 | 1/1 | 2/2 | |
| 2.6 | 1/1 | 1/1 | |
| D KOSR, C KOSR | 5.3 | – | 2/2 |
| 5.45 | – | 2/2 | |
F1 129S6;C57BL/6N ESCs were derived (D) in and cultured (C) under various conditions. Chimeric mice were generated by different means (described in table headings) and then tested for ESC germline contribution. Results show the number of mice demonstrating germline transmission out of the number of mice tested per condition.