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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Atherosclerosis. 2016 Jun 9;251:109–118. doi: 10.1016/j.atherosclerosis.2016.06.011

Figure 3. Single injection of AAV-mPCSK9 mutant (D377Y) induces vascular calcification in high-fat, high-cholesterol fed C57BL/6J mice.

Figure 3

A) Ex vivo FRI analysis. Representative images of the fluorescence intensity in the aorta. Red squares (200x200 pixel) indicate the quantified area including aortic sinus and arch. B) Quantification of FRI signal-positive area (dashed red line indicates signal cut-off for quantification). **p < 0.01, ***p < 0.001 by one-way ANOVA. C, D) Representative images of tissue non-specific alkaline phosphatase activity (TNAP stain) in the aortic sinus (C) and aortic arch (lesser curvature) (D). C, Bar=200 µm C; D, Bar=100 µm. (E) Quantitative assessment of %-positive lesion area for TNAP. Data presented as mean±SD. Each dot depicts one mouse. n.s., not significant. F) Representative images of von Kossa stain for calcium deposits in the aortic arch (lesser curvature). Bar=100 µm. n=3–8 for all panels.