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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Arch Toxicol. 2016 Apr 22;90(9):2231–2248. doi: 10.1007/s00204-016-1711-1

Fig. 1.

Fig. 1

Lung fibrosis induced by MWCNT. Wild-type C57BL/6J mice received saline, DM vehicle, carbon black (40 μg per mouse) or MWCNT (40 μg per mouse) and were killed on day 7 post-exposure. A Total collagen fibers were detected by Masson’s Trichrome staining of lung tissue sections, with collagen fibers stained blue (a–d scale bar 50 μm). Collagen I and III fibers were visualized by Picro-Sirius Red staining, with collagen fibers stained red (e–h scale bar 50 μm). B Collagen I (a–d) and FN1 (e–h) protein expression was determined by immunohistochemistry on lung sections. Red indicates positive staining, and blue indicates nuclear counterstaining (scale bar 20 μm)