Fig. 4.

p2 introgression interval identified on chromosome 20. Positions are given for polymorphic SoySNP50K markers in the genomic region. The p2 allele, from the donor line T31, was previously backcrossed into the recurrent parents ‘Clark’ and ‘Harosoy’ to generate five Near Isogenic Lines (NILs) as part of the Soybean Isoline Collection. The recurrent parent genotypes are color coded in blue, and the donor parent genotypes are color coded in red, heterozygous (Het.) genotypes are color coded in blue and red, and missing genotypes are color coded in gray. Heterozygous scores are likely due to heterogeneity in the NIL. Examination of the five p2 NILs’ genotypes identified a single introgression interval (566 kb) in the genome in which all five lines shared the donor parent genotype (Gm20:1,582,950–2,148,735). This interval contains the GmNAP1 gene (Gm20:1,999,216–2,021,765) (color figure online)