Fig 3.

Effects of cell seeding density and dynamic culture on cell reprogramming. A. Orbital shaking applied at different time points as indicated by the graph on the left, with the corresponding reprogramming efficiency indicated on the right. (*p<0.05. n=3.) B. Reprogramming efficiencies after reseeding cells on day 6. Fibroblasts were initially seeded at different densities and, on day 6, either reseeded to a lower density of 10000/cm² (grey bars) or reseeded without any adjustment in cell density (white bars). (*p<0.05. n=3.) C. Reprogramming efficiencies for static and dynamic culture at different initial seeding densities. Dynamic culture was started on day 6 onwards. (*p<0.05. n=3.) D. Cells were seeded at low seeding densities (500 and 1000/cm²), and reprogrammed under static or dynamic culture (starting at day 6). (*p<0.05. n=3.)