Table 4.
The most frequent sequencing targets used for identification of nontuberculous mycobacteria.
| Target gene | Name | Primer type | Sequence 5′ → 3′ | Length of the product (bp) | Reference |
|---|---|---|---|---|---|
| 16S rRNA | 16S27f 16S907r |
Forward Reverse |
AGAGTTTGATCMTGGCTCAG CCGTCAATTCMTTTRAGTTT |
921 | Harmsen et al., 2003 [12] |
|
| |||||
| hsp65 | Tb11 Tb12 |
Forward Reverse |
ACCAACGATGGTGTGTCCAT CGGATCACTCGTGAACGCTA |
441 | Telenti et al., 1993 [13] |
|
| |||||
| ITS | 16S-1511f 23S-23r |
Forward Reverse |
AAGTCGTAACAAGGTARCCG TCGCCAAGGCATCCACC |
Approx. 380∗ | Harmsen et al., 2003 [12] |
|
| |||||
| rpoB | Myco-F Myco-R |
Forward Reverse |
GGCAAGGTCACCCCGAAGGG AGCGGCTGCTGGGTGATCATC |
764 | Adékambi et al., 2003 [14] |
∗Amplicon size is not uniform due to the 16S-23S spacer length variability.