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. Author manuscript; available in PMC: 2017 Oct 15.
Published in final edited form as: Mol Cell Endocrinol. 2016 Jun 17;434:116–126. doi: 10.1016/j.mce.2016.06.020

Figure 3. Transduction of GCs with constitutively active FOXO1 confirms that FOXO1 represses or activates FSH gene targets.

Figure 3

GCs were transduced overnight with Ad-E, Ad-WT-FOXO1 (Ad-WT), or constitutively active Ad-FOXO1(A3), media was replaced and 5 hours later cells were treated with vehicle or FSH (50 ng/ml) for 24 hours, as described in Materials and Methods. mRNA expression of indicated genes, relative to Rpl19 and expressed as 2−ΔCt, was determined as described in Materials and Methods. Results are means ± SEM of 3 independent experiments. Statistical significance was determined by one-way ANOVA followed by Tukey’s multiple comparisons test. Different letter designations for each gene indicate significantly different groups (P<0.05).