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. 2016 Aug 15;7:1168. doi: 10.3389/fpls.2016.01168

FIGURE 2.

FIGURE 2

Isolation of protoplasts from leaf tissue by enzymatic method. (A) Digested holm oak leaf tissues after 4 h at 25°C (scale bar length: 6 mm); (B) Mesophyll protoplasts of holm oak from fourth leaf (scale bar length: 10 μm); (C) Mesophyll protoplasts of white poplar (scale bar length: 10 μm).