Table 3.
Genetic parameters from the amplification of DNA from metal‐contaminated and uncontaminated sites using methylation‐sensitive primers
| Genetic parameters | Uncontaminated | Metal‐contaminated |
|---|---|---|
| Number of primer sets | 8 | 8 |
| Number of loci per primer set | 46 | 46 |
| Number of polymorphic loci | 284 | 266 |
| Percentage of polymorphic loci | 76.55 | 71.70 |
| Observed number of alleles/epialleles per locus (na) | 1.7655 ± 0.42 | 1.717 ± 0.45 |
| Effective number of alleles/epialleles per locus (ne) | 1.3264 ± 0.33 | 1.3254 ± 0.34 |
| na‐ne | 0.4391 | 0.3916 |
| Nei's (1973) gene diversity (He) | 0.2026 ± 0.18 | 0.2 ± 0.18 |
| Shannon's information index (I) | 0.3183 ± 0.25 | 0.3112 ± 0.26 |
| Mean genetic/epigenetic distance | 0.2405 | 0.2395 |
| Minimum genetic/epigenetic distance | 0.0000 | 0.0000 |
| Maximum genetic/epigenetic distance | 0.3868 | 0.3632 |
| Relative degree of genetic/epigenetic diversity (Gst) | 0.0865 | 0.0962 |
| Gene flow (Nm) | 5.2807 | 4.698 |
| Polymorphic information content (PIC) | 0.1745 | 0.1728 |
| Neutrality | 0.734762 | 0.740847 |
| Number (%) of non‐neutral sites | 0.265238 | 0.259153 |