Figure 4.

The effect of TLR2 knockout (down) on IL-15 expression in intestinal epithelial cells. (a) RT-PCR analysis of IL-7 and IL-15 expression in the jejunum sample. RNA was prepared from the samples of the TLR2^−/− mice and the wild-type mice. (b) Immunohistochemistry with IL-15 antibody on sections of small intestine from wild-type mice + PBS, wild-type mice + lipoteichoic acid (LTA), TLR2^−/− mice + PBS, and TLR2^−/− mice + LTA. Images are 400x. (c) SW480 cells were transfected with TLR2-siRNA or Mock and TK-RL and pBIIx-luc. At 24 h after transfection, the cells were stimulated with the indicated concentrations of LTA, and then both firefly and Renilla luciferase activities were determined using a dual-luciferase assay. The experiments were repeated three times, and all of the data were expressed as the mean ± SD. ^∗∗ P < 0.01; ^∗∗∗ P < 0.001.