Fig. S4.

Callus formation in VLCFA-deficient mutants. (A) Schematic representation of the fatty acid elongase complex. VLCFAs are biosynthesized by the elongase complex composed of KCS, KCR, HCD (also known as PAS2), and ECR. (B) RT-PCR analysis of PAS1 expression in WT and pas1-4 (SALK_051324) plants. (C) Callus-forming phenotype of WT, pas1-4, slr, and pas1-4 slr plants. Phenotypic and cytological characterizations were performed with 7-d-old seedlings incubated on CIM for 12 d (Upper) or 4 d (Lower), respectively. (Scale bars: 1 cm in Upper, 50 µm in Lower.) (D and E) Callus-forming phenotype in the hypocotyl (D) and cotyledon (E) of WT, kcs1-2, and pas1-4 plants. (Scale bars: 1 mm.)