Fig. S7.

VLCFA-mediated pericycle competence does not rely on cytokinin or ethylene homeostasis. (A and B) ALF4 transcription is not responsive to cytokinin or AgNO₃ treatment. Here 7-d-old WT and pALF4::GUS seedlings were treated with or without (Mock) 2 µM kinetin or 10 µM AgNO₃ for 6 h, respectively. n = 3. Error bars are SD. (Scale bar: 50 µm.) (C and D) Callus formation (Upper) and cytological characteristics (Lower) of transgenic p35S::IPT3 (C) and p35S::CKX1 kcs1-2 (D) seedlings on CIM. (Scale bars: 1 cm in Upper, 50 µm in Lower.) (E and F) Effect of AgNO₃ or ACC on pericycle competence for callus formation. Callus-forming phenotype of 7-d-old WT seedlings on CIM with or without (Mock) 10 µM AgNO₃ (E) or kcs1-2 seedlings on CIM with or without 10 µM ACC (F) for 12 d. (Scale bars: 1 cm.)