Fig. S7.

Effect of Tet2/3 DKO on the Igκ rearrangement repertoire. Vκ contribution to the Igκ repertoire was analyzed from RNA-seq data on follicular B cells from three wt and three Tet2/3⁻ mice. RPKM of each Vκ segment was normalized to the sum of all Vκ reads to give a percent of the repertoire. Vκ segments which contribute less than 0.4% of the repertoire in all samples were removed from the analysis to overcome outlier effects of lowly expressed genes. The ratio between the wt and Tet2/3⁻ contribution to the repertoire for each Vκ segment is presented. Error bars represent SEM. The distal V segments (blue) are down-regulated in Tet2/3⁻, whereas the proximal Vs (red) are up-regulated (P < 10⁻⁵).