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Nonphosphorylatable mutants do not reduce phosphorylation of other available PKC substrates. (A) 32P (Top) and Western blot (WB) of immunoprecipitation of Syt1 in WT neurons with and without stimulation with PDBu. (B) As in A, using munc18-1 KO neurons rescued with WT or nonphosphorylatable Munc18. (C) Quantification of 32P blots in B, normalized to the WB signal. n = 3.
