Fig. S5.

Structure models and theoretical R_H values. Structure models for R_H calculation were generated based on the crystal structures of the Rep_1–198/Ant_1–86 complex and the Rep_92–198 tetramer and were used for comparison with those of analytical gel filtration (Fig. 4B). The two invisible NDDs of the Rep_1–198/Ant_1–86 complex were incorporated by superimposing visible NDDs. The Rep_1–198 dimer model was obtained from the Rep_1–198/Ant_1–86 complex without modifying the NDD positions. To obtain the overall model of the full-length Rep tetramer, NDDs were positioned by superimposing full-length Rep on the Rep_92–198 tetramer.