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Detection of T1 and T2 B cells. Flow cytometry of IgMhiCD23− T1 and IgMhiCD23+ T2 subsets within B220+CD19+CD93+ transitional B cells in the spleens of Mb1-cre−, Mb1-cre+, and Nemofl Mb1-cre+ mice in the absence of Bcl2 (A; n = 5–7 per genotype in six experiments) or upon ectopic expression of Bcl2 (Bcl2Tg) (B; n = 14–19 per genotype in 15 experiments). Numbers adjacent to outlined areas specify the percentage of cells in the gate.
