Allelic expression of 27-kDa γ-zein gene in different lines. (A) XF134 expressing a shorter 27-kDa γ-zein. (B) An 18-bp deletion in XF134-γ27. (C) Allelic examination of 27-kDa γ-zein gene expression in XF134, B73, K0326Y, and Mo17. Because the endosperm is triploid (two from the female and one from the male), the cDNA numbers of two 27-kDa γ-zein alleles are expected to be 2:1 (expectation rate), if the parent lines don’t have qγ27 or qγ27 acts in a trans manner. Superscript “a” represents χ20.05 = 3.84.