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Copy-number variation and gene expression. (A) Southern blot of 27-kDa γ-zein gene in B73, Mo17, and K0326. (B) Quantitative expression analysis of 27-kDa γ-zein gene, GRMZM2G138976, and GRMZM5G873335 in B73 and Mo17. Because Mo17 contains the duplication, RNA transcript levels of the three genes are from their two copies. **P < 0.01.
