| C70A |
Active site |
Structural stabilization of active site by a disulfide bridge |
| R119A |
N-terminal insertion |
Arg119 is involved in salt-bridge interactions allowing N-terminal insertion in BsrV |
| R121A |
N-terminal insertion |
Arg121 is involved in salt-bridge interactions allowing N-terminal insertion in BsrV |
| A165K |
Active site |
Ala at this position allows the correct stabilization of the Cl− ion by Arg173 and Asn174 (in Alrs, this position is occupied by the N-carboxylated Lys) |
| N167A |
Active site |
Structural stabilization of loop involved in Cl− coordination |
| G169A |
Active site |
Loop involved in Cl− coordination |
| N174A |
Active site |
Residue involved in Cl− coordination |
| P206N |
Entry loops |
Structural stabilization of the L1 loop (involved in substrate entry) |
| Y208A |
Entry loops |
Stabilization of the PLP phosphate group |
| K216A |
Entry loops |
Salt-bridge interaction stabilizing α10 at the entry |
| Y246A |
Entry loops |
Mutation will block movement of the r2 region |
| G263I |
Active site |
Gly residue allows Asp268 and Tyr394 stabilization of side chains of different substrates |
| N348A |
Active site |
Stabilization of loop that includes Met347 at the catalytic site |
| T349A |
Active site |
| P391N |
Active site |
This Pro is critical in the broad specificity of BsrV, allowing the stabilization of side chains from different substrates |
