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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Connect Tissue Res. 2016 Apr 29;57(4):319–333. doi: 10.1080/03008207.2016.1182996

Figure 7.

Figure 7

Histology and immunohistochemistry. Safranin-O/Fast green staining shows greater accumulation of GAG in auricular chondrocyte aggregates differentiated at 20% O2 vs. 5% O2; the reverse is true for the articular cartilage aggregates. Collagen type II staining (E–H) shows no apparent difference in the auricular chondrocyte aggregates but is clearly stronger in articular chondrocyte aggregates at 5% O2 vs. 20% O2. Collagen type X staining (I–L) is stronger in auricular chondrocyte aggregates at 20% O2 vs. 5% O2 and is more matrix associated. In articular chondrocyte aggregates, there is a slightly stronger staining at 5% O2 vs. 20% O2. Elastin staining (M–P) was weak across all samples with a slightly greater accumulation at 20% O2 for the auricular chondrocyte aggregates only.