FIG 3.

Model-based prediction of growth of P. pastoris in glucose-limited aerobic retentostat cultures. An optimized feed profile, designed to achieve a smooth decline of the specific growth rate (μ) from 0.025 h⁻¹ to near-zero growth rates, was designed by computer simulation (see Materials and Methods). Indicated are the optimized glucose concentration profile in the feed (short dashed line), the resulting predicted profiles of biomass accumulation (long dashed line), the specific glucose uptake rate (q_S) (solid line), and μ (dotted line).